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目的通过对苏皖地区健康体检者的血清总IgE(tIgE)和特异性IgE(sIgE)水平进行测定,分析探讨其临床价值及意义。方法本研究采用酶联免疫捕获法和荧光免疫法,分别对苏皖地区接受健康体检的379例志愿者(男207例,女172例,17~64岁,中位数27岁)行血清tIgE和sIgE测定。血清tIgE水平≤100 k U/L为阴性,>100 k U/L为阳性;sIgE采用吸入性变应原过筛试验(Phadiatop),<0.35 k U/L(0级)为阴性,≥0.35 k U/L(1级以上)为阳性。使用SPSS 18.0统计软件分析数据,P<0.05表示差异有统计学意义,组间比较进行矫正(α=0.05/6=0.008 3)。结果 379例健康志愿者中,血清tIgE水平介于1.48~1 903.22(中位数53.70)k U/L,男性显著高于女性(P<0.001),其中tIgE阳性和阴性者分别为129例(34.04%)和250例(65.96%);血清sIgE水平范围为0.01~45.20(中位数0.07)k U/L,男女之间无明显差异(P=0.207),其中sIgE阳性和阴性者分别为88例(23.22%)和291例(76.78%)。进一步分析,四个年龄组中血清tIgE水平在17~30岁年龄组最高(中位数62.18 k U/L,P=0.006),且男性tIgE水平在该年龄组最高(中位数80.60 k U/L,P=0.009)。血清sIgE水平在四个年龄组中无明显差异(P=0.318)。血清sIgE阳性人群中有67例(76.14%)tIgE水平>100 k U/L,而sIgE阴性人群中有62例(21.31%)tIgE水平>100 k U/L。血清tIgE阴性人群中sIgE阴性者占绝对多数(91.60%,P<0.001)。Spearman秩相关分析显示,血清tIgE与sIgE水平的相关系数为0.650,呈正相关(P<0.001)。结论苏皖地区健康成人的血清tIgE与sIgE水平具有较强的的相关性。低水平的血清tIgE(100 k U/L以下)有助于排除变态反应(变应原致敏),可作为一个临床参考指标。
Objective To determine the serum total IgE (tIgE) and specific IgE (sIgE) levels in healthy subjects in Jiangsu and Anhui Provinces, and to explore the clinical value and significance. Methods In this study, 379 volunteers (207 males, 172 females, 17-64 years old, median 27 years old) undergoing physical examination in Jiangsu and Anhui Provinces were enrolled in this study. Serum tIgE And sIgE assay. Serum tIgE level ≤100 kU / L was negative,> 100 kU / L was positive; sIgE by inhalation allergen screening test (Phadiatop), <0.35 kU / L (level 0) was negative, ≥ 0.35 k U / L (level 1 or above) is positive. Data were analyzed using SPSS 18.0 statistical software, with a P <.05 indicating a statistically significant difference between the groups (α = 0.05 / 6 = 0.008 3). Results Among 379 healthy volunteers, serum tIgE levels ranged from 1.48 to 1 903.22 (median 53.70) kU / L, significantly higher in males than in females (P <0.001), with 129 cases of tIgE positive and negative 34.04%) and 250 cases (65.96%). Serum sIgE levels ranged from 0.01 to 45.20 (median 0.07) kU / L, with no significant difference between men and women (P = 0.207) 88 cases (23.22%) and 291 cases (76.78%). Further analysis, serum tIgE levels among the four age groups were highest in the 17 to 30 age group (median, 62.18 kU / L, P = 0.006), and tIgE levels were highest in this age group (median 80.60 kU /L,P=0.009). Serum sIgE levels did not differ significantly among the four age groups (P = 0.318). Sixty-seven (76.14%) of the serum sIgE-positive patients had a tIgE level of> 100 kU / L compared to 62 (21.31%) of the sIgE-negative patients with a tIgE level of> 100 kU / L. Negative sIgE in the serum tIgE negative population accounted for the absolute majority (91.60%, P <0.001). Spearman rank correlation analysis showed that the correlation coefficient between serum tIgE and sIgE level was 0.650 (P <0.001). Conclusion The serum levels of tIgE and sIgE in healthy adults in Jiangsu and Anhui Provinces are strongly correlated. Low levels of serum tIgE (up to 100 kU / L) can help eliminate allergic reactions (allergen sensitization) and can be used as a clinical reference.