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一、材料:500ml三角瓶,手提式高压灭菌锅。菌种上海平菇,凤尾菇PL-27。培养基配方:20％土豆煮汁,2％白糖,0.15％硫酸镁,0.3％磷酸二氢钾,2％蛋白胨,0.04％琼脂,水1000ml,pH7-7.5。二、试验方法:500ml三角瓶装上述配方的培养液150-200ml,常规灭菌后在接种箱内接入供试的平菇、凤尾菇斜面母种约黄豆大小一块,置恒温箱内27-28℃下静止培养24小时,外观无杂菌污染,菌块开始萌发。这时,每天开始手摇振荡3次,每次
First, the material: 500ml flask, portable autoclave. Strain Shanghai mushroom, mushroom mushroom PL-27. Medium recipe: 20% potato boiled juice, 2% sugar, 0.15% magnesium sulfate, 0.3% potassium dihydrogen phosphate, 2% peptone, 0.04% agar, water 1000 ml, pH 7-7.5. Second, the test method: 500ml flask filled with the formula of the culture medium 150-200ml, routine sterilization in the inoculation box access to the test mushrooms, mushrooms beveled slope type about a soybean, set the incubator 27- 28 ℃ static culture for 24 hours, the appearance of no bacterial contamination, bacteria began to germinate. At this time, start shaking 3 times a day, each time