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观察了白细胞介素４（ＩＬ－４）基因转移的肿瘤细胞体内致瘤性的变化。构建了含５００ｂｐ的小鼠ＩＬ－４ｃＤＮＡ的真核表达载体ＢＭＧＮｅｏ－ＩＬ－４．用该表达载体将ＩＬ－４基因转移至小鼠Ｂ１６黑色素瘤细胞，通过Ｇ４１８抗性筛选、有限稀释法及ＩＬ－４活性检测，获得了高分泌ＩＬ－４的黑色素瘤细胞克隆株并经Ｎｏｒｔｈｅｒｎ印迹鉴定，将其接种于小鼠皮下后观察到肿瘤生长缓慢、小鼠存活期延长，表明高分泌ＩＬ－４的细胞克隆株体内致瘤性显著下降。该结果为进一步研究肿瘤的ＩＬ－４基因治疗打下了基础。
The in vivo tumorigenicity of tumor cells transfected with interleukin 4 (IL-4) gene was observed. An eukaryotic expression vector BMGNeo-IL-4 containing 500 bp mouse IL-4 cDNA was constructed. IL-4 gene was transferred to mouse B16 melanoma cells using this expression vector. Melanoma cell clones with high secretion of IL-4 were obtained by G418 resistance screening, limiting dilution assay, and IL-4 activity detection. After imprinting, the mice were inoculated subcutaneously in the mice and the growth of the tumor was slow and the survival period of the mice was prolonged. This indicates that the tumorigenicity of the cell clones with high IL-4 secretion was significantly reduced in vivo. This result laid the foundation for further study of tumor IL-4 gene therapy.