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本实验采用放射免疫测定法（ＲＩＡ）测定了成人牙周炎（ＡＰ）、青少年牙周炎（ＪＰ）、龈炎（Ｇ）和健康人（Ｈ）（各１０例）龈组织中６－酮－前列腺素Ｆｉａ（６－Ｋ－ＰＧＦ＿１ａ）的含量，用免疫组织化学ＡＢＣ染色对同一标本中的６－Ｋ－ＰＧＦ＿１ａ定位，用显微分光光度计（ＭＳＰ）对染色标本定量，与ＲＩＡ结果比较，以探讨６－Ｋ－ＰＧＦ＿１ａ与牙周病的关系。ＲＩＡ结果表明：牙周病患者龈组织中６－Ｋ－ＰＧＦ１ａ含量从Ｈ、Ｇ、ＡＰ到ＪＰ呈递增趋势，各病变组与Ｈ皆有显著差异，ＪＰ与Ｇ组有显著差异，ＡＰ与Ｐ也有显著差异。ＡＢＣ染色表明龈组织中６－Ｋ－ＰＧＦ＿１ａ主要的分泌细胞为血管内皮细胞、巨噬细胞、浆细胞、多形核白细胞及个别的腺管内皮细胞等。对牙龈组织６－Ｋ－ＰＧＦ＿１ａ的ＭＳＰ定量结果与ＲＩＡ相似，两方法可相互补充。提示对６－Ｋ－ＰＧＦ＿１ａ与牙周病关系的研究有重要意义。
In this study, 6-keto-2-methyl-2-pyrimidine-2-carboxymethylcellulose was measured by radioimmunoassay (RIA) in adult periodontitis (AP), adolescent periodontitis (JP), gingivitis (G) and healthy human - Prostaglandin Fia (6-K-PGF_1a) levels were determined by immunohistochemical ABC staining of 6-K-PGF_1a in the same specimen. The stained specimens were quantified with a micro-spectrophotometer (MSP) To investigate the relationship between 6-K-PGF_1a and periodontal disease. The results of RIA showed that the content of 6-K-PGF1a in gingival tissues of patients with periodontal disease increased gradually from H, G, AP to JP, and there was a significant difference between each lesion group and H, there was significant difference between JP and G group There are also significant differences. ABC staining showed that the main secreting cells of 6-K-PGF_1a in gingival tissues were vascular endothelial cells, macrophages, plasma cells, polymorphonuclear leukocytes and some endothelial cells. MSP quantitative results for gingival tissue 6-K-PGF_1a are similar to RIA, and the two methods complement each other. Tip 6-K-PGF_1a and periodontal disease is of great significance.