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目的:采用一测多评,建立槐角中异黄酮类成分的含量测定方法。方法:采用Agilent Extend C18色谱柱(250 mm×4.6mm,5μm),以甲醇-乙腈-0.1%磷酸水溶液为流动相,流速为1.0 mL·min-1,柱温为30℃,检测波长为260 nm,建立槐角苷与槐属双苷、染料木苷和染料木素的相对校正因子,并以相对保留时间作为目标峰的定位标准。结果:色谱峰分离度良好,槐角苷在0.374～7.49μg、槐属双苷在2.16×10-2～0.432μg、染料木苷在2.61×10-2～0.522μg、染料木素在1.02×10-2～0.203μg呈良好线性关系,平均加样回收率槐角苷为100.7%、槐属双苷为96.3%、染料木苷为98.6%、染料木素为100.8%。槐角苷对槐属双苷、染料木苷和染料木素的相对校正因子分别为0.80、1.03、1.61。相对保留时间分别为0.42、0.81、1.26。结论:采用一测多评法测定槐角中异黄酮类成分的含量是准确、可行的。
OBJECTIVE: To establish a method for the determination of isoflavones in Huaijiaojiao by using multiple tests. Methods: An Agilent Extend C18 column (250 mm × 4.6 mm, 5 μm) was used with mobile phase of methanol-acetonitrile-0.1% phosphoric acid as mobile phase at a flow rate of 1.0 mL · min-1 with a column temperature of 30 ℃ and a detection wavelength of 260 nm, the relative correction factor between acacia and geniposide, genistin and genistein was established, and the relative retention time was used as the standard for the target peak. Results: The resolution of chromatographic peaks was good with the ranges of 0.374 ~ 7.49 μg for sophoridine, 2.16 × 10-2 ~ 0.432 μg for sophoricoside, 2.61 × 10-2 ~ 0.522 μg for genistin and 1.02 × for genistein 10-2 ~ 0.203μg showed a good linear relationship, the average recovery of the recovery rate of 100.7% of acacia, sophora glycosides 96.3%, genistein 98.6%, genistein 100.8%. The relative correction factors of acacia glycosides, genistein and genistein were 0.80, 1.03 and 1.61 respectively. Relative retention time was 0.42,0.81,1.26. Conclusion: It is accurate and feasible to determine the content of isoflavones in Huaijiaojuan by using a multi-evaluation method.