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目的:通过对大鼠给予不同致呕吐物质的刺激,研究大鼠在接受不同呕吐物质刺激后其延髓组织5-羟色胺(5-HT)的变化,为中医学“呕吐”证发生的病理病机“胃气上逆”的理论,提供分子生物学依据。方法:Wistar健康成年大鼠32只,随机分为4组,即对照1组,藜芦组,对照2组,顺铂组,每组8只。对照1组以生理盐水20mL/kg灌胃;藜芦组给予藜芦浓缩液灌胃,计量为20mL/kg,每毫升浓缩液相当与0.28g生药;对照2组腹腔注射生理盐水10mL/kg,顺铂组以浓度1mg/1mL顺铂注射液10mL/kg腹腔注射。给药6h后进行标本采集,取大鼠延髓。采用高效液相色谱法检测大鼠延髓组织中5-HT含量。采用免疫组化法检测5-HT表达的变化。结果:与对照1组相比藜芦组大鼠延髓组织5-HT含量增高(P<0.05),表达上调(P<0.05)。结论:藜芦可使大鼠延髓组织5-HT含量增高,表达上调。“胃气上逆”与延髓组织5-HT含量增加有关。
OBJECTIVE: To study the changes of 5-hydroxytryptamine (5-HT) in the medulla oblongata of rats after stimulating with different vomit substances through the stimulation of different vomit substances in rats, which is the pathology of TCM “vomiting” Pathogenesis “stomach upside down ” theory, provide molecular biology basis. Methods: Thirty-two Wistar healthy adult rats were randomly divided into 4 groups: control group 1, veratridine group, control group 2 and cisplatin group, with 8 in each group. Control group 1 with saline 20mL / kg gavage; veratridine group given veratridine concentrates by gavage, measured as 20mL / kg, equivalent to 0.28g per ml of concentrate and crude drugs; control group 2 intraperitoneal injection of saline 10mL / kg, Cisplatin group with concentration of 1mg / 1mL cisplatin injection 10mL / kg intraperitoneal injection. Specimens were collected 6h after administration, and medulla oblongata was taken. The contents of 5-HT in the medulla oblongata of rats were detected by high performance liquid chromatography. The expression of 5-HT was detected by immunohistochemistry. Results: Compared with the control group 1, the content of 5-HT in the medulla oblongata was increased (P <0.05) and the expression was increased (P <0.05). Conclusion: Veratridine can increase the content of 5-HT in medulla oblongata and upregulate it. “Stomach upside down ” and the bulbar 5-HT content increased.