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首次将生物发酵破壁技术应用于中草药提取。利用药用白腐真菌Lentinu edodes AD发酵甘草后,甘草酸得率较水提法增加5.25%,所得甘草提取物浸膏中甘草酸的相对含量增加24.4%,提取后固体废弃物排放量也显著下降。同时,该技术在甘草废渣的资源利用上具有一定的潜力,发酵甘草废渣后甘草酸得率为21.8 mg/g。通过卷烟加香试验表明,生物提取法得到的甘草浸膏在卷烟中的加香效果强于水提法得到的甘草浸膏。因此,白腐菌生物提取法是一种新型的、具有选择性和环境友好的植物药提取方法。
The first fermentation of biological fermentation technology applied to Chinese herbal medicine extraction. The yield of glycyrrhizic acid increased by 5.25% compared with that of water extract after the fermentation of licorice by white rot fungus Lentinu edodes. The relative content of glycyrrhizic acid in the extract of licorice extract increased by 24.4%, and the discharge of solid waste after extraction was also significant decline. At the same time, the technology has certain potential in resource utilization of licorice residue, and the yield of glycyrrhizic acid after fermentation of licorice residue is 21.8 mg / g. The experiment of flavoring with cigarettes showed that the flavoring effect of licorice extract obtained by biological extraction was stronger than that of licorice extract obtained by water extraction. Therefore, the white rot fungus biological extraction method is a new, selective and environmentally friendly method for the extraction of botanicals.