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目的观察微波消融对131I-肿瘤细胞核人鼠嵌合单克隆抗体(131I-chTNT)在小鼠体内分布的影响。方法将45只小鼠分为A、B、C组,建立H22肝癌模型后,分别予以131I-chTNT瘤内单点注射、微波消融后消融范围中心注射131I-chTNT、微波消融后消融范围以外的肿瘤组织注射131I-chTNT。注药后5 d,采用γ计数仪测定小鼠不同器官(肿瘤、血、心、肝、胃、肝、肾)每克组织的131I-chTNT摄取率;注药后3 d,行小鼠肿瘤组织切片的放射自显影及HE染色,观察131I-chTNT在小鼠瘤内分布情况。结果与A组相比,B、C组每克肿瘤组织131I-chTNT摄取率明显升高(P均<0.05),肿瘤组织内坏死区域明显增大,药物在瘤内的分布增加。结论微波消融可增加131I-chTNT在肿瘤组织内的浓聚,与注药部位无关。
Objective To observe the effect of microwave ablation on the distribution of 131I-tumor nuclear human murine chimeric monoclonal antibody (131I-chTNT) in mice. Methods Forty-five mice were divided into group A, B, and C. After the H22 liver cancer model was established, 131I-chTNT single intratumoral injection, microwave ablation followed by 131I-chTNT in the center of ablation range, and ablation range after microwave ablation were performed. Tumor tissue was injected with 131I-chTNT. Five days after the injection, the 131I-chTNT uptake rates per gram of tissue in different organs (tumor, blood, heart, liver, stomach, liver, and kidney) of mice were measured using a gamma counter; mice were tumorigenic at 3 days after injection. The tissue sections were autoradiographed and HE stained to observe the distribution of 131I-chTNT in mouse tumors. Results Compared with group A, the 131I-chTNT uptake rate per gram of tumor tissue in groups B and C was significantly increased (P<0.05). The area of necrosis in the tumor tissue was significantly increased, and the distribution of the drug in the tumor was increased. Conclusion Microwave ablation can increase the concentration of 131I-chTNT in tumor tissue, which is not related to the injection site.