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目的 :对腐败尸体不同组织扩增效果进行比对。方法 :将腐败尸体的毛发 (带毛囊 )、肌肉、骨头、软骨、血液 (血纱 )用Chelex -1 0 0的方法进行消化提取DNA ,荧光标记复合扩增 ,扩增产物用荧光DNA自动测序仪进行分离和检测。结果 :不同的腐败组织扩增效果不相同 ,以软骨的扩增效率为高。结论 :针对不同情况提取检材 ,特别是提取软骨组织对日常检案非常重要。
Objective: To compare the amplification effect of different tissues of corrupted corpse. Methods: The DNA of the hair (hair follicle), muscle, bones, cartilage and blood (blood yarn) of the corpse was digested by Chelex -100 method and amplified by fluorescent labeling. The amplified product was automatically sequenced by fluorescent DNA Instrument for separation and testing. Results: The effects of different corrupt tissue amplification were different, and the efficiency of cartilage expansion was high. Conclusion: It is very important for the routine examination to extract the samples according to different conditions, especially to extract the cartilage tissue.