引言 自本世纪六十年代,印度科学家S.Guha在蔓陀萝上取得花粉植株以来,许多植物的花药培养均取得了成功,通过花药培养,得到了小麦,水稻等新品种。花药培养已成为获得新品种的一条途径。柑桔花药培养可为柑桔遗传育种研究提供许多有价值的材料。日本首先在枳壳上得到花粉植株。随后,我国在四季桔、雪柑等柑桔种上也培养出花粉植株。由于花药培养受环境因素,如气候、树体生理状况等的影响较大,因此有必要研究在我国的环境条件下,枳壳花粉植株的再生技术,以便在育种实践中更好地利用这一抗寒资源。 Introduction Since the 60s of this century, the Indian scientist S.Guha has obtained the anther culture of many plants since the pollen plants were obtained on the Manduca oleracea. New varieties of wheat and rice have been obtained through anther culture. Anther culture has become a means of obtaining new varieties. Citrus anther culture can provide many valuable materials for citrus genetic breeding research. Japan first got pollen plants on Citrus aurantium. Subsequently, our country in the four seasons orange, citrus and other citrus species also cultivate pollen plants. As the anther culture is affected by environmental factors, such as climate and physiological status of trees, it is necessary to study the regenerative technology of Citrus aurantium pollen plants under the environmental conditions in our country in order to make better use of this anther in breeding practice Cold resources.