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目的:探讨华蟾素注射液对H 22荷瘤小鼠体内β-内啡肽的影响。方法:24只昆明小鼠右腋皮下注射小鼠H 22肝癌细胞建立荷瘤模型,随机分设为模型组和华蟾素组,另设正常组,每组12只。用ELISA法测定各组小鼠肿瘤周围组织(正常组在相同部位)和血浆中的β-内啡肽的含量。结果:华蟾素组的小鼠肿瘤周围组织中的β-内啡肽的含量高于模型组和对照组,差异具有显著性;而在血浆中,模型组的小鼠的β-内啡肽的含量高于华蟾素组和正常组。结论:华蟾素注射液能够显著的升高H 22荷瘤小鼠肿瘤周围组织中β-内啡肽的含量,而对其血浆中的含量影响较小。
Objective: To investigate the effect of cinobufacini injection on β-endorphin in H22 tumor-bearing mice. METHODS: Twenty-four Kunming mice were injected subcutaneously into the right axilla of H22 hepatoma cells to establish a tumor-bearing model. The mice were randomly divided into model group and cinobufotalin group, with 12 normal mice in each group. The content of β-endorphin in the peri-tumor tissue (normal group at the same site) and plasma were determined by ELISA. Results: The content of β-endorphin in periarterion tissue of cinobufotalin group was higher than that of model group and control group, and the difference was significant. In plasma, β-endorphin Higher than cinobufacini group and normal group. CONCLUSION: Cinobufacini injection can significantly increase the content of β-endorphin in the peritumoral tissue of H22 tumor-bearing mice, but has little effect on its plasma content.