用硫酸水解试样,再过滤、稀释水解产物。产物经氯胺T氧化后,与对二甲氨基苯甲醛反应生成红色化合物,用分光光度计进行比色测定。本法测定“皮革奶”中L-羟脯氨酸的含量可操作性强,适合批量检测。 The sample is hydrolyzed with sulfuric acid, filtered, and the hydrolyzate is diluted. The product was oxidized with chloramine T and reacted with p-dimethylaminobenzaldehyde to generate a red compound. The colorimetric determination was performed with a spectrophotometer. Determination of the Law “leather milk ” L- hydroxyproline content operability, suitable for batch testing.