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目的:优选芪丹颗粒的提取工艺。方法:以丹酚酸B、丹参酮ⅡA质量分数和干膏率为评价指标,通过正交试验考察乙醇体积分数、提取次数、乙醇用量对醇提工艺的影响;以黄芪甲苷提取量和出膏率为指标,通过正交试验考察煎煮时间、煎煮次数、加水量对水提取工艺的影响。采用HPLC测定黄芪甲苷、丹酚酸B及丹参酮ⅡA含量。结果:最佳醇提工艺为加6倍量60%乙醇回流提取2次,提取时间分别为2,1 h;丹酚酸B和丹参酮ⅡA质量分数分别为1.215%,0.599%,干膏率41.04%。最佳水提工艺为加10倍量水煎煮2次,每次1.5 h;黄芪甲苷提取量3.408 mg·g-1,出膏率43.37%。结论:优选的提取工艺稳定可行,为芪丹颗粒的合理开发提供参考。
Objective: To optimize the extraction process of Qi Dan granules. Methods: Taking salvianolic acid B, tanshinone ⅡA mass fraction and dry cream rate as evaluation indexes, the effects of ethanol volume fraction, extraction times and ethanol dosage on alcohol extraction process were investigated by orthogonal test. Rate as an indicator, through orthogonal test to investigate the boiling time, boiling times, the amount of water on the water extraction process. Astragaloside IV, salvianolic acid B and tanshinone IIA were determined by HPLC. Results: The optimal alcohol extraction process was extracting twice with 6 times of 60% ethanol and extracting for 2 hours and 1 hour respectively. The contents of salvianolic acid B and tanshinone ⅡA were 1.215%, 0.599% and 41.04, respectively %. The best water extraction process was adding 10 times the amount of boiling water twice, each 1.5 h; Astragaloside extraction 3.408 mg · g-1, the rate of 43.37% cream. Conclusion: The optimal extraction process is stable and feasible, which provides a reference for the rational development of Qidan granules.