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目的分析长春地区丙型肝炎病毒(HCV)基因型的分布及其与HCV RNA含量、感染途径、肝病程度的相关性。方法采用荧光定量PCR和型特异性引物PCR对82份抗-HCV阳性病人的血清标本进行HCV RNA检测及HCV的基因分型,并分析基因型与感染途径、HCV RNA含量和肝病程度的相关性。结果78份HCV RNA阳性血清标本中1a型占5.1%、1b型占48.7%、2a型占33.3%、2b型占11.6%、3a型占1.3%,未发现混合型。血源性与非血源性感染患者间HCV基因型构成比差异无统计学意义。基因1b型的HCV RNA含量高于2a型,且差异有统计学意义。HCV感染的不同程度肝病患者的基因型分布差异有统计学意义,基因1b型比率显著高于其他基因型。结论长春地区丙型肝炎病毒基因型流行株为1b和2a型;HCV基因型与感染途径无明显相关性;基因lb型的病毒含量明显高于2a型;HCV基因型与慢性丙型肝炎的严重程度及肝硬化、肝癌的产生有一定的相关性。
Objective To analyze the distribution of hepatitis C virus (HCV) genotypes in Changchun and its correlation with the content of HCV RNA, the path of infection and the degree of liver disease. Methods The serum samples of 82 anti-HCV positive patients were detected by real-time polymerase chain reaction (PCR) and genotyping primers (PCR). The HCV RNA was detected and the genotypes of HCV were analyzed. The genotypes were also compared with those of infection, HCV RNA content and severity of liver disease . Results Among the 78 HCV RNA positive sera, type 1a accounted for 5.1%, type 1b accounted for 48.7%, type 2a accounted for 33.3%, type 2b 11.6% and type 3a 1.3%. No mixed type was found. There was no significant difference in the constituent ratio of HCV genotype between patients with and without blood-borne infection. HCV genotype 1b genotype higher than the content of 2a, and the difference was statistically significant. There were significant differences in the genotype distribution between patients with HCV infection and other liver diseases. The ratio of genotype 1b was significantly higher than that of other genotypes. Conclusions The genotypes of HCV genotypes 1b and 2a in Changchun are not obvious. The genotypes of HCV and infection have no obvious correlation. The genotype 1b virus is significantly higher than genotype 2a. The genotypes of HCV and chronic hepatitis C are serious Degree and cirrhosis, liver cancer have a certain correlation.