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以初始平均体重为(12.5±2.0)g的刺参为研究对象,在室内玻璃钢桶内进行了56 d饲喂实验。以基础饲料为对照组(A),研究基础饲料中分别添加0.5%(B)、1.25%(C)、2.0%(D)的裂壶藻对刺参生长、免疫及消化酶的影响。结果表明,C组、D组可显著提高刺参的特定生长率(SGR)(P<0.05)。C组和D组刺参体腔液碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、溶菌酶(LZM)、超氧化物歧化酶(SOD)、吞噬活性、肠道淀粉酶均显著高于对照组(P<0.05)。C组的体腔液呼吸爆发活性和肠道蛋白酶活性显著高于对照组(P<0.05)。B组的AKP、LZM、肠道淀粉酶活性均显著高于对照组(P<0.05)。饲料中添加裂壶藻各处理组刺参成活率均为100%。实验结果表明,1.25%-2.0%裂壶藻添加量可显著提高刺参的生长速度和免疫酶活性;饲料中添加1.25%裂壶藻能够显著增加刺参肠道的蛋白酶、淀粉酶的消化活性:裂壶藻有作为刺参营养添加剂的应用前景。
The initial average body weight of (12.5 ± 2.0) g of sea cucumber as the research object, in the indoor fiberglass drum 56 days feeding experiment. The basal diet was used as the control group (A) to study the effects of schizonepeta on the growth, immunity and digestive enzymes of sea cucumber (Apostichopus japonicus) with 0.5% (B), 1.25% (C) and 2.0% The results showed that group C and group D significantly increased the specific growth rate (SGR) of sea cucumber (P <0.05). The alkaline phosphatase (AKP), acid phosphatase (ACP), lysozyme (LZM), superoxide dismutase (SOD), phagocytic activity and intestinal amylase in C group and D group were significantly higher than those in A group Control group (P <0.05). Body cavity fluid respiration burst activity and intestinal protease activity in group C were significantly higher than those in control group (P <0.05). The AKP, LZM and intestinal amylase activities in group B were significantly higher than those in control group (P <0.05). The survival rate of sea cucumber was 100% for each treatment group. The results showed that the 1.25% -2.0% Pythium algae addition rate can significantly improve the growth rate and immune enzyme activity of sea cucumber; addition of 1.25% Pythium can significantly increase the digestive activity of protease, amylase : Crataegus have the application prospect as the sea cucumber nutrient additive.