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本文对慢性脱氧麻黄素（MethamphetamineMeth；1mg／kg／day2—8周）染毒大鼠心肌中热休克蛋白的代表物HeatshockProtein70（HSP70）和Ubiquitin进行了免疫组织化学的研究。实验结果显示早期Meth染毒阶段（2周组），虽然心肌组织未出现明显的病理形态学变化，但在心肌细胞、心内膜上皮细胞、血管平滑肌细胞和毛细血管内皮细胞内均可检出HSP70和Ubiqui－tin。以后随着染毒时间的延长，HSP70和Ubiquitin在细胞内的表达也增强。而对照组大鼠心肌组织在实验期间缺乏明显的表达。这些发现提示在受到Meth作用时，HSP70和Ubiquitin在心肌细胞内的表达优先于病理形态学改变的出现。且石蜡组织切片中热休克蛋白的免疫组织化学研究是早期诊断心肌细胞损伤的一个良好方法。
In this study, immunohistochemistry of Heat shock protein 70 (HSP70) and Ubiquitin, a heat shock protein in myocardium of rats exposed to methotrexate (Methamphetamine Methyl; 1 mg / kg / day 2-8 weeks) The experimental results showed that in the early stage of Meth exposure (2 weeks group), although there was no obvious pathomorphological changes in myocardial tissue, it could be detected in myocardial cells, endocardial epithelial cells, vascular smooth muscle cells and capillary endothelial cells HSP70 and Ubiqui-tin. With the extension of exposure time, HSP70 and Ubiquitin expression in the cell also increased. Myocardial tissue in the control group lacked significant expression during the experiment. These findings suggest that the expression of HSP70 and Ubiquitin in cardiomyocytes takes precedence over the appearance of pathological changes when exposed to Meth. Immunohistochemistry of heat shock proteins in paraffin sections is a good way to diagnose cardiomyocyte injury in the early stage.