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采用纯漆酶与介体2,2-连氮-双-(3-乙基苯并噻吡咯啉-6-磺酸)(简称ABTS)组成的漆酶/ABTS介体系统对低浓度吲哚进行降解实验。综合考虑,选取降解条件为pH 3、ABTS 0.5 mmol/L,漆酶添加量1 U/mL时,对低浓度吲哚溶液的降解效果最佳,降解率达到80%以上。介体ABTS的加入对降解率的提高发挥了重要作用。体系中决定吲哚降解率高低的重要因素为pH和介体浓度,漆酶添加量影响较小。在HPLC谱图2.4 min和2.7 min出现的新物质可能是氧化吲哚和靛红。
The laccase / ABTS mediator system consisting of pure laccase and mediator 2,2-azin-bis- (3-ethylbenzothiopyrroline-6-sulfonic acid) Degradation experiments. Taken together, the optimal degradation conditions were pH 3, ABTS 0.5 mmol / L and laccase dosage 1 U / mL. The degradation efficiency of indole solution with low concentration was the best, and the degradation rate reached more than 80%. The addition of mediator ABTS played an important role in the degradation rate. The important factors that determine the level of indole degradation in the system are pH and mediator concentration, but laccase dosage is less affected. New substances that appear in the 2.4 min and 2.7 min HPLC chromatograms may be indoxyl and isatin.