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本文首次提出一种简便、快速地同时测定配合饲料中多种水溶性维生素的反相离子对HPLC方法。此方法用合适的波长和灵敏度程序检测,以抑制和排除杂质峰的干扰;在样品预处理上,采取特殊措施以防止维生素的分解,减少杂质含量。
This paper presents for the first time a simple and rapid simultaneous determination of water-soluble vitamins in feed compound by RP-HPLC method. This method uses a suitable wavelength and sensitivity program to detect and prevent interference with impurity peaks. In sample preparation, special precautions should be taken to prevent the breakdown of vitamins and to reduce the amount of impurities.