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已发现老年患者吞噬细胞的胞内杀灭作用降低或无改变,胞外细胞毒作用降低,但摄入功能无改变或增强。为阐明老年人吞噬细胞这种Fcγ受体功能改变的机制,作者研究了健康青年(18～25岁)和老年(60～85岁)男性多形核白细胞(PMNL)在吞噬已调理的酵母菌过程中环核苷酸的变化。 PMNL用密度离心法分离,剩余红细胞用低渗盐水裂溶三次,可得纯度95％和活性97％的细胞。培基用RPMI1640,细胞浓度为5×10~6/ml,每ml加经新鲜人血清调理加热杀死的酵母菌5×10~7,37℃60分钟后离心、涂片、染色,计数200个细胞的吞噬菌数。用放免法药盒检测吞噬过程中不同时间(0、15、30、60和120分)时的细胞内cAMP和cGMP水平,对照为不加吞噬刺激的PMNL。
It has been found that phagocytic cells in elderly patients with or without intracellular killing to reduce the extracellular cytotoxicity decreased, but no change in intake or enhancement. To elucidate the mechanisms by which Fcγ receptors are altered in phagocytes in the elderly, we examined the effect of phagocytosis of conditioned yeast (PMNL) by healthy young (18-25 years) and elderly (60-85 years) male polymorphonuclear leukocytes (PMNL) Cyclic nucleotide changes in the process. PMNL was separated by density centrifugation, the residual erythrocytes were lysed three times with hypotonic saline to obtain cells with 95% purity and 97% activity. Culture medium with RPMI1640, the cell concentration of 5 × 10 ~ 6 / ml, each ml plus fresh human serum conditioning heat to kill the yeast 5 × 10 ~ 7,37 ℃ 60 minutes after centrifugation, smear, staining, counting 200 Number of phagocytic cells. The levels of intracellular cAMP and cGMP at different time points (0, 15, 30, 60 and 120 minutes) of phagocytosis were detected by radioimmunoassay kit, and the control was PMNL without stimulation of phagocytosis.