组织培养液具备适合于哺乳类细胞生长的各种营养成份。主要有无机盐,氨基酸和维生素组成。多数还须加入血清才能生长,血清的机制尚不十分明了。不含血清的化学综合培养基可适合于某些异倍体细胞的生长。许多学者曾创立了一些适于某特定细胞生长的培养液,所有培养液共同特点是给微生物提供了极好的生长条件。 在组织培养操作过程中,如接种或再培养均需打开容器,培养的温度又适宜,极易造成污染。何况离体细胞不如在机体中具有物理或化学的防御机能。例如人类皮肤等防止感染,就具有下列特点:①干燥表皮细胞;②多层化;③表层不断脱落;④pH在4.0—6.0,平均4.85;⑤表面披以油质,防止浸湿与浸溶;⑥角质化防止擦伤;⑦披以表 Tissue culture fluid has a variety of nutrients suitable for mammalian cell growth. Mainly inorganic salts, amino acids and vitamins. Most also have to join the serum to grow, the mechanism of serum is not yet clear. Non-serum chemical synthetic medium may be suitable for the growth of some aneuploid cells. Many scholars have created some suitable for the growth of a particular cell culture medium, all the common characteristics of the culture medium is to provide microorganisms with excellent growth conditions. In the process of tissue culture operation, such as inoculation or re-culture need to open the container, culture temperature is appropriate, easily lead to pollution. Not to mention ex vivo cells in the body as a physical or chemical defense function. Such as human skin to prevent infection, has the following characteristics: ① dry epidermal cells; ② multi-layered; ③ surface off continuously; ④ pH 4.0-6.0, an average of 4.85; ⑤ surface covered with oil to prevent wetting and leaching; ⑥ keratinization to prevent abrasions; ⑦ Phi table