论文部分内容阅读
目的：研究膳食钙和ＶＤ对大鼠脂质代谢的影响。方法：以Ｗｉｓｔａｒ雄性大鼠为实验对象建立高脂动物模型，按血清胆固醇水平并参考体重将动物分为五组：Ａ组，０．０８％Ｃａ；Ｂ组，０．５％Ｃａ；Ｃ组，０．５％Ｃａ，同时补充ＶＤ；Ｄ组，１．６％Ｃａ；Ｅ组，１．６％Ｃａ，同时补充ＶＤ。Ｃ、Ｅ组以维生素Ｄ３油溶液灌胃（１５μｇ／ｋｇｂｗ），每周两次。实验持续６５天。结果：实验结束时，Ｄ组血清胆固醇和甘油三酯水平显著低于Ａ、Ｂ组，Ｂ组又显著低于Ａ组。Ｃ组的血脂水平与Ｂ组相比无显著性差异，Ｅ组较Ｄ组显著升高。各组的组织脂质含量与血脂水平平行。Ｄ组的粪脂和粪钙含量显著高于Ａ、Ｂ组，Ｂ组又显著高于Ａ组。Ｃ组与Ｂ组相比，Ｅ组与Ｄ组相比，粪脂和粪钙含量皆显著降低。结论：膳食高钙通过减少肠道脂质的吸收而降低血脂水平和组织中的脂质含量。ＶＤ促进钙的吸收而对脂质代谢产生影响，在０．５％Ｃａ基础上补充一定剂量的ＶＤ对于脂质代谢无不良影响；在１．６％Ｃａ的基础上补充同样剂量的ＶＤ对脂质代谢产生不良的影响。
Objective: To study the effects of dietary calcium and VD on lipid metabolism in rats. Methods: The animal model of high fat was established in Wistar male rats. The animals were divided into five groups according to serum cholesterol level and body weight: group A, 0.08% Ca, group B, 0.5% Ca, group C , 0.5% Ca, while VD; D group, 1.6% Ca; E group, 1.6% Ca, while VD. Groups C and E were gavaged with vitamin D3 oil solution (15μg / kg bw) twice a week. The experiment lasted 65 days. Results: At the end of the experiment, serum cholesterol and triglyceride levels in group D were significantly lower than those in groups A and B, and group B was significantly lower than group A. There was no significant difference in lipid level between group C and group B, and group E was significantly higher than group D. The lipid levels of the tissues in each group were parallel to the lipid level. D group of fecal fat and fecal calcium content was significantly higher than the A, B group, B group was significantly higher than the A group. Compared with group B, the content of fecal fat and fecal calcium in Group C and Group D were significantly lower than those in Group B. CONCLUSIONS: Dietary high calcium reduces lipid levels and lipid levels in the tissues by reducing intestinal lipid absorption. VD to promote the absorption of calcium on lipid metabolism, supplemented with a certain dose of VD on the basis of 0.5% Ca has no adverse effect on lipid metabolism; on the basis of 1.6% Ca added the same dose of VD on lipid Metabolism has an adverse effect.