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目的:建立大黄通气口服液中大黄酸、大黄素及大黄酚的HPLC含量测定方法。方法:采用Inertsil ODS-SP(4.6mm×150 mm,5μm)色谱柱,流动相甲醇-0.1%磷酸(72∶28),柱温25℃,检测波长440 nm,流速1.0 mL·min-1。结果:测定大黄酸、大黄素及大黄酚的线性范围分别为4.2~50.4(r=0.999 9),3.9~46.8,(r=0.999 7),4.2~50.4 mg·L-1(r=0.999 9);平均回收率(n=5)为大黄酸97.68%,RSD 1.09%,大黄素97.36%,RSD 59%,大黄酚97.27%,RSD 0.97%。结论:方法简便、结果准确、重复性好,可用于该制剂的质量控制。
Objective: To establish a HPLC method for determination of rhein, emodin and chrysophanol in rhubarb ventilation oral solution. Methods: Inertsil ODS-SP (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was methanol-0.1% phosphoric acid (72:28). The detection wavelength was set at 440 nm and the flow rate was 1.0 mL · min-1. Results: The linear ranges of rhein, emodin and chrysophanol were 4.2 ~ 50.4 (r = 0.999 9), 3.9 ~ 46.8 (r = 0.999 7), 4.2 ~ 50.4 mg · L -1 (r = 0.999 9 ). The average recovery (n = 5) was 97.68% for rhein, 1.09% for RSD, 97.36% for emodin, 59% for RSD, 97.27% for chrysophanol and 0.97% for RSD. Conclusion: The method is simple, accurate, reproducible and can be used for the quality control of the preparation.