论文部分内容阅读
目的高效液相色谱法测定云厚朴生品及3种炮制品中4种活性成分的含量,考察炮制对云厚朴主要化学成分的影响,为云厚朴资源的合理开发利用提供依据。方法高效液相色谱法测定含量,色谱条件为:戴安U3000系列高效液相色谱仪(配备PDA检测器);Agilent SB-C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相:采用甲醇-0.1%冰醋酸梯度洗脱;流速:1.0 mL/min;检测波长:紫丁香苷为265 nm,丁香酚为280 nm,厚朴酚及和厚朴酚为294 nm;柱温:25℃,提取方法为甲醇超声提取。结果所建立的4种活性成分含量测定方法平均加样回收率范围97.21%~98.36%,RSD在1.16%~1.43%范围内。4种活性成分的含量由高到低顺序为和厚朴酚、厚朴酚、紫丁香苷和丁香酚,但姜炙品中紫丁香苷含量低于丁香酚含量,生品及炮制品的厚朴酚及和厚朴酚的总含量满足2015年版《中国药典》的要求。结论所建立的云厚朴生品及3种炮制品中4种活性成分含量测定方法准确、可靠。炮制方法的不同,对云厚朴药材活性成分的含量造成不同程度的影响。
OBJECTIVE To determine the contents of four active ingredients in raw materials of Magnolia officinalis and three processed products by high performance liquid chromatography (HPLC) and investigate the influence of processing on the main chemical components of Magnolia officinalis so as to provide the basis for the rational development and utilization of the resources. Methods HPLC was used to determine the content. The chromatographic conditions were as follows: Diane U3000 series high performance liquid chromatograph with PDA detector; Agilent SB-C 18 column (4.6 mm × 250 mm, 5 μm); mobile phase : Gradient elution with methanol-0.1% glacial acetic acid; flow rate: 1.0 mL / min; detection wavelength: syringin at 265 nm, eugenol at 280 nm, honokiol and honokiol at 294 nm; 25 ℃, extraction method for methanol ultrasonic extraction. Results The average recoveries of four active ingredients were 97.21% ~ 98.36%, RSD was in the range of 1.16% ~ 1.43%. The contents of 4 active ingredients were honokiol, honokiol, syringin and eugenol in descending order, but the content of syringin in ginger was lower than that of eugenol. The thickness of raw and processed products The total content of pakchoi and honokiol satisfies the requirements of the 2015 edition of “Chinese Pharmacopoeia”. Conclusions The method for determining the content of four active ingredients in Yun-huang Pu-sheng and its three processed products is accurate and reliable. Different processing methods, the content of active ingredients of Yun Magnolia caused varying degrees of influence.