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背景与目的NOK是新近发现的属于RPTKs家族成员癌基因,可促进细胞转化、肿瘤发生、侵袭和转移,但NOK蛋白缺乏膜受体结构,通过何种胞膜受体传导信号而被激活及在非小细胞肺癌(NSCLC)中的表达仍不清楚。本研究通过检测NOK和EGFR蛋白在NSCLC中的表达,分析其表达与病理分类、分级和TNM分期关系以及NOK与EGFR表达的相关性,为进一步研究NOK在肿瘤发生发展中的作用机制提供依据。方法采用免疫组化EnVinsion法检测NOK和EGFR在155例NSCLC中的表达,用统计学分析表达差异性和蛋白间的相关性。结果NOK阳性表达主要定位于细胞胞质中,EGFR定位于胞膜和胞质中。NOK、EGFR蛋白在鳞癌、腺癌中高表达,与相应正常组织表达相比差异明显(P<0.001)。鳞癌、腺癌组间相比NOK、EGFR的表达无统计学差异(P=0.099;P=0.23),但NOK、EGFR在鳞癌、腺癌不同病理分级和TNM不同分期中的表达差异明显(P<0.01)。NOK、EGFR在总的NSCLC及其组织分类的鳞癌、腺癌中的表达率和阳性强度存在相关性(P<0.001),相关系数rs分别为0.742、0.722、0.756。结论NOK在NSCLC中高表达,表达的高低与病理分级及TNM分期有关,可能与NSCLC的发生发展有关;NOK与EGFR蛋白在NSCLC及其组织分类中表达存在很好的相关性,NOK信号通路的激活可能与EGFR有关,这为进一步研究NOK在肿瘤发生发展中的作用及其机制提供重要依据。
Background and Objective NOK is a recently discovered oncogene belonging to members of the RPTKs family which promotes cell transformation, tumorigenesis, invasion and metastasis. However, NOK protein lacks the membrane receptor structure and is activated by which cell membrane receptor-transducing signal and at The expression in non-small cell lung cancer (NSCLC) remains unclear. This study examined the expression of NOK and EGFR protein in NSCLC to analyze the relationship between the expression of the protein and pathological classification, grading and TNM staging, as well as the relationship between NOK and EGFR expression, to provide a basis for further study on the mechanism of NOK in tumorigenesis. Methods Immunohistochemical EnVionion method was used to detect the expression of NOK and EGFR in 155 cases of NSCLC. Statistical analysis was used to analyze the correlation between the expression and protein. Results The positive expression of NOK was mainly located in the cytoplasm of cells, and EGFR was located in the membrane and cytoplasm. NOK, EGFR protein was highly expressed in squamous cell carcinoma and adenocarcinoma, which was significantly different from the corresponding normal tissue (P <0.001). There was no significant difference in the expression of EGFR between squamous cell carcinoma and adenocarcinoma (P = 0.099; P = 0.23), but there were significant differences in the expression of NOK and EGFR in different stages of squamous cell carcinoma, adenocarcinoma and TNM (P <0.01). There was a significant correlation between the expression of EGFR and the positive rate of NOK and EGFR in squamous cell carcinomas and adenocarcinomas (P <0.001). The correlation coefficients rs were 0.742, 0.722, 0.756 respectively. Conclusions NOK is highly expressed in NSCLC. The expression of NOK is related to the pathological grade and TNM stage, which may be related to the development of NSCLC. There is a good correlation between the expression of NOK and EGFR protein in NSCLC and its tissue classification. The activation of NOK signaling pathway Which may be related to EGFR, which provide an important basis for further study of the role of NOK in tumorigenesis and its mechanism.