论文部分内容阅读
1概述1．1测量依据：JJG705-2002《液相色谱仪检定规程》1．2环境条件：温度(10～30)℃,8小时内温度波动不超过±3℃相对湿度20%～85%RH 1．3测量标准：GBW(E)130167～130170液相色谱检定标准物质1．4被测对象：液相色谱仪1．5测量过程：选用C18色谱柱,以100%甲醇为流动相,流量为1．0ml/min,紫外检测器的波长选在254nm,检测灵敏度调到最灵敏档,开机预热,待仪器稳定后,用微量注射器从进样口注入10-20μL1×110~(-7)g/mL的荼-甲醇,按规程公式计算最小检测浓度
1 Overview 1.1 Measurement basis: JJG705-2002 "liquid chromatography test procedures 1.2 Environmental conditions: temperature (10 ~ 30) ℃, 8 hours temperature fluctuations of not more than ± 3 ℃ Relative humidity 20% to 85% RH 1.3 Measurement standards: GBW (E) 130167 ~ 130170 HPLC standard material 1.4 The measured object: liquid chromatography 1.5 Measurement process: the choice of C18 column with 100% methanol as the mobile phase, The flow rate of 1.0ml / min, UV detector wavelength selection at 254nm, detection sensitivity transferred to the most sensitive file, boot warm, until the instrument is stable, with a micro syringe injected from the inlet 10-20μL1 × 110 ~ 7) g / mL of tea - methanol, according to the formula to calculate the minimum test concentration