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【目的】探讨神经干细胞(NSCs)增殖和分化过程中过氧化物酶增殖物激活受体γ(PPARγ)的表达情况及意义。【方法】体外分离培养神经干细胞,诱导其分化,先用免疫荧光法查看PPARγ在NSCs中的定位,然后分别取未分化的及诱导分化后d1、d5、d9的NSCs,应用RT-PCR和Western Blot法检测各相应时间点NSCs内PPARγ的mRNA及蛋白表达。【结果】成功培养大量未分化呈巢状悬浮生长的神经干细胞团,并诱导其分化为神经元和神经胶质细胞。PPARγ蛋白存在于NSCs细胞核内,并在NSCs未分化阶段高水平表达,NSCs诱导分化后则表达量随时间延长而逐渐下降。【结论】在NSCs的增殖阶段,PPARγ的表达较高,而在分化过程中,表达量逐渐减少,提示PPARγ可能对NSCs的增殖和分化起重要作用。
【Objective】 To investigate the expression and significance of peroxisome proliferator-activated receptor γ (PPARγ) during the proliferation and differentiation of neural stem cells (NSCs). 【Methods】 Neural stem cells were isolated and cultured in vitro to induce their differentiation. The expression of PPARγ in NSCs was observed by immunofluorescence method. Then, the undifferentiated and differentiated NSCs were detected by RT-PCR and Western Blot method was used to detect the mRNA and protein expression of PPARγ in NSCs at each time point. 【Results】 A large number of NSCs were cultured successfully in undifferentiated nests and induced to differentiate into neurons and glial cells. PPARγ protein is present in the nucleus of NSCs and expressed at high level in the undifferentiated stage of NSCs. The expression of PPARγ protein gradually decreased with the passage of time after NSCs differentiation. 【Conclusion】 The expression of PPARγ is higher in the proliferative phase of NSCs, and gradually decreased in the differentiation process, suggesting that PPARγ may play an important role in the proliferation and differentiation of NSCs.