目的:优化酶法提取大黄总蒽醌工艺。方法:以大黄总蒽醌浓度为评价指标,用单因素方差分析法研究酶剂量、酶解温度、酶解时间和pH对提取大黄总蒽醌的影响;采用效应面法,以酶解大黄所提取的总蒽醌浓度为指标,对酶法提取大黄总蒽醌工艺条件进行优化。结果:酶剂量为0.12 g时,总蒽醌含量最高(3.1730±0.1023 mg/L),且大黄总蒽醌浓度随着酶解时间的增加而增加,并以酶解48 h蒽醌浓度最高(2.1410±0.1252 mg/L);此外,pH在4.8、酶解温度在50℃时,大黄总蒽醌浓度最高分别达到2.2777±0.2133 mg/L和4.1360±0.3877 mg/L。以效应面法优化酶法提取大黄总蒽醌的最佳工艺为:pH:4.59,酶剂量:0.1 g,酶解温度:45.86℃。结论:该工艺条件易于控制、工艺简单,成本低,在此条件下大黄酶解后总蒽醌的最高浓度为4.3231 mg/L。 Objective: To optimize enzymatic extraction of rhubarb total anthraquinone. Methods: The total anthraquinone concentration of rhubarb was used as an index to evaluate the effects of enzyme dosage, enzymolysis temperature, enzymolysis time and pH on the extraction of total anthraquinone from rhubarb. The response surface methodology The extracted total anthraquinone concentration as an index, the enzymatic extraction Rhubarb anthraquinone process conditions are optimized. Results: The total anthraquinone content was the highest (3.1730 ± 0.1023 mg / L) at 0.12 g enzyme dosage, and the total anthraquinone concentration increased with the increase of enzymolysis time, and reached the peak at 48 h 2.1410 ± 0.1252 mg / L). In addition, the maximum total anthraquinone concentrations in Rhubarb reached 2.2777 ± 0.2133 mg / L and 4.1360 ± 0.3877 mg / L, respectively, at a pH of 4.8 and a hydrolysis temperature of 50 ℃. The optimum conditions for enzymatic extraction of Rhizoma Total anthraquinone by the response surface methodology were: pH 4.59, enzyme dosage 0.1 g, enzymatic temperature 45.86 ℃. Conclusion: The process conditions are easy to control, the process is simple, and the cost is low. Under this condition, the highest total anthraquinone concentration of Rhizoma was 4.3231 mg / L.