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利用ＯＳ５－ＦＬ叶绿素荧光仪研究干旱胁迫下蔗叶叶绿体荧光参数的变化及其与甘蔗耐旱性关系。应用干旱胁迫下蔗叶活性氧代谢变化等耐旱鉴定指标鉴定参试品种的相对耐旱性。结果表明，干旱胁迫下甘蔗生长受抑制，蔗叶叶绿素含量、相对含水量、叶绿体ＤＣＩＰ光还原活性下降，ＭＤＡ含量和质膜相对透性提高；叶绿体荧光参数Ｆｖ／Ｆｍ、Ｆｖ／Ｆｏ、Ｔ１／２均明显降低，表明干旱胁迫使蔗叶光系统Ⅱ（ＰＳⅡ）原初光能转换效率、ＰＳⅡ潜在活性受到抑制；研究还表明干旱胁迫下蔗叶叶绿体光合量子产额（Ｙｉｅｌｄ）及其潜在活性（Ｆｄｓ／Ｆｓ）降低，说明干旱胁迫直接影响了光合作用的电子传递和二氧化碳同化过程。上述荧光参数的受抑程度因品种抗逆性能而异，不抗旱品种降幅明显高于抗旱品种。最后讨论了叶绿素荧光动力学在甘蔗抗旱育种及抗旱鉴定上应用的可能性。
Study on the Change of Chloroplast Fluorescent Parameters and the Drought Tolerance of Sugarcane under Drought Stress by OS5 - FL Chlorophyll Fluorescence. The relative drought tolerance index of drought-tolerant cultivars was determined by drought tolerance index of active oxygen metabolism of sugarcane leaves under drought stress. The results showed that under drought stress, the growth of sugarcane was inhibited, chlorophyll content, relative water content of sugarcane leaves, photoreduction activity of chloroplast DCIP decreased, MDA content and the relative permeability of plasma membrane increased. The chlorophyll fluorescence parameters Fv / Fm, Fv / Fo, T1 / 2 were significantly decreased, indicating that the primary light energy conversion efficiency of PSⅡ and the potential activity of PSⅡ were inhibited under drought stress. The study also showed that under drought stress, the photosynthetic quantum yield of chloroplast and its potential activity Fds / Fs) decreased, indicating that drought stress directly affects the photosynthesis electron transfer and carbon dioxide assimilation process. The suppression of the above fluorescence parameters due to the variety of resistance to different performance, no decline in drought-resistant varieties was significantly higher than drought-resistant varieties. Finally, the possibility of applying chlorophyll fluorescence kinetics to drought resistance breeding and drought resistance identification of sugarcane was discussed.