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近几年来,由于鲎试验不能证明伤寒患者血浆中存在内毒素,故对伤寒发病的内毒素刺激学说发生了怀疑;同时,内毒素类似抗原抗体复合物对人类白细胞的作用和免疫复合物引起伤寒肾脏并发症问题,益受重视.本文测定31例伤寒患者血清免疫复合物(IC)及补体C_3,并讨论其意义.材料与方法(一)血清来源采自31例伤寒患者,其中普通型30例.感染性休克死亡1例;男17例,女14例.(二)测定方法IC测定用酶联免疫吸附试验(ELISA),操作步骤按文献2方法,用EDTA分段沉淀法提取人血清中的C_(1q),选择适量C_(1q),包被微量反应板,加稀释待检血清孵育后,加辣根过氧化物酶,标记马抗人IgG,以邻苯二胺为底物,在72型分光光度计的492nm处测OD,后者0.3以上判为阳性.30例正常
In recent years, due to 鲎 test can not prove the presence of endotoxin in the plasma of patients with typhoid, so the theory of endotoxin stimulation of typhoid fever was suspected; at the same time, endotoxin-like antigen-antibody complex on human leukocytes and immune complexes cause typhoid Kidney complications, benefit should be valued.In this paper, 31 cases of typhoid patients serum immunocomplex (IC) and complement C_3, and discuss its significance.Materials and Methods (a) serum from the 31 cases of typhoid patients, of which 30 Cases of septic shock in 1 case; 17 males and 14 females (b) Determination of IC Determination of enzyme-linked immunosorbent assay (ELISA), steps according to the literature method 2, EDTA segmented precipitation of human serum In the C_ (1q), select the appropriate amount of C_ (1q), coated microtiter plate, diluted serum to be seized after incubation, add horseradish peroxidase, horse anti-human IgG labeled with phenylenediamine as substrate , Measured at 492nm at 72 spectrophotometer OD, which was judged as positive 0.3 or above .30 normal