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目的 观察重复过敏原吸入刺激致敏的大鼠气道细胞DNA合成和气道壁结构的改变 ,并探讨气道重塑反应发生的机制。方法 应用SD大鼠建立哮喘模型 ,采用胶原染色、粘液染色和双标免疫组化染色等技术对气道细胞的DNA合成和气道重塑进行研究。结果 (1)模型组和正常组相比 ,气道上皮下胶原组织大量沉积 ,上皮表面粘液层增厚 ,杯状细胞增生 ,并有大量炎性细胞浸润 ;(2 )模型组气道平滑肌细胞DNA合成表达Brdu阳性核计数为 10 3± 2 1,明显高于正常对照组 (7 2±2 1) ,(t=3 6 6 ,P <0 0 1) ;模型组气道上皮细胞的DNA合成表达Brdu阳性核计数为 2 1± 7,亦明显高于正常对照组 (16± 4 ) (t=2 35 ,P <0 0 5 )。结论 (1)重复过敏原刺激可使气道细胞DNA合成增加 ,上皮下胶原沉积和粘液分泌增加。 (2 )变态反应性炎症所致的微环境改变可能是导致气道重塑反应发生的主要机制
Objective To observe the changes of DNA synthesis and airway wall structure of sensitized rat airway cells induced by repeated allergen inhalation and to explore the mechanism of airway remodeling. Methods Sprague-Dawley rats were used to establish the model of asthma. The collagen synthesis, airway remodeling and airway remodeling were studied by collagen staining, mucus staining and double immunohistochemical staining. Results (1) Compared with the normal group, the collagen deposition in the airway epithelium was massive in the model group, the thickening of the mucous layer on the epithelial surface, the goblet cell hyperplasia and the infiltration of a large number of inflammatory cells. (2) The expression of DNA in the airway smooth muscle cells The positive expression of Brdu in nucleus was 103 ± 21, which was significantly higher than that of the normal control group (72 ± 21) (t = 366, P <0.01). The DNA synthesis of airway epithelial cells The positive Brdu expression was 21.7%, which was also significantly higher than that of the normal control group (16.4%) (t = 35, P <0.05). Conclusions (1) Repeated allergen stimulation can increase airway cell DNA synthesis, subepithelial collagen deposition and mucus secretion. (2) Alteration of microenvironment caused by allergic inflammation may be the main mechanism leading to airway remodeling reaction