离心沉降分析技术是分子生物学基本实验技术之一。3年来,我们用Model-282分析型超速离心机测定了蛋白质、核酸及多糖的分子量(M,沉降-扩散法)和沉降系数(S)80多次,均获得满意结果,为鉴定这些生物大分子的理化特性提供基本数据,本文对该技术原理、方法及测定实例作详细介绍。实验表明,它是测定生物大分子特性的简便、精确、可靠的基准方法。 Centrifugal sedimentation analysis technology is one of the basic experimental techniques of molecular biology. For 3 years, we have obtained satisfactory results with Model-282 analytical ultracentrifuge for molecular weight (M, sedimentation-diffusion) and sedimentation coefficient (S) of protein, nucleic acid and polysaccharide for many times. The physical and chemical properties of molecules to provide basic data, this paper describes the principle of the technology, methods and examples of measurement in detail. Experiments show that it is a simple, accurate and reliable method for determining the characteristics of biological macromolecules.