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我们建立了垂体组织块短时温育并施加电场刺激的离体实验体系，运用此方法并结合放射免疫测定激素含量，观察了大鼠垂体前叶内神经纤维对促肾上腺皮质激素（ＡＣＴＨ）释放的影响。结果表明，电场刺激能够促使垂体前叶ＡＣＴＨ释放显著增加，刺激参数为强度３０ｍＡ，波宽０．５ｍｓ，频率１０Ｈｚ。这个效应可为温育液中加入河豚毒素（ＴＴＸ）和藜芦碱所阻断，但ＴＴＸ不能阻断精氨酸加压素（ＡＶＰ）诱发的ＡＣＴＨ分泌。同样参数的电场刺激对分散培养的大鼠垂体前叶细胞ＡＣＴＨ的分泌没有显著作用。以上结果说明，我们所用参数的电场刺激产生的效应是兴奋了垂体前叶内的神经纤维，而非直接刺激腺细胞所致。上述结果提示：垂体前叶激素分泌的调节除了传统的体液途径之外，还可能存在直接的神经控制。
We established an in vitro experimental system in which pituitary tissue blocks were incubated for a short period of time and applied electric field stimulation. Using this method combined with radioimmunoassay, we observed the effects of nerve fibers in the anterior pituitary on the release of adrenocorticotropic hormone (ACTH) Impact. The results showed that the stimulation of electric field can promote the anterior pituitary ACTH release increased significantly, the stimulation parameters of intensity 30mA, wave width 0.5ms, frequency 10Hz. This effect was blocked by addition of tetrodotoxin (TTX) and veratridine to the incubation solution, but TTX did not block arginine-vasopressin (AVP) -induced ACTH secretion. The same parameters of electric field stimulation on dispersed cultured rat anterior pituitary ACTH secretion no significant role. The above results indicate that the effect of the electric field stimulation of the parameters we use is excitement of nerve fibers in the anterior pituitary gland rather than directly stimulating glandular cells. The above results suggest that the regulation of anterior pituitary hormones may have direct neuronal control in addition to the traditional humoral pathway.