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为探讨氧自由基引发红细胞膜脂质过氧化对红细胞膜流动性的影响在红细胞氧化损伤机制中的作用，以氧自由基发生系统（ＦｅＣｌ２／抗坏血酸）作用于离体正常红细胞，分别用荧光偏振法、硫代巴比妥酸比色法测定红细胞膜微粘度、红细胞脂质过氧化物（ＬＰＯ）。结果表明：氧自由基发生系统作用于红细胞后，红细胞ＬＰＯ增加［（６．１８１±０．２１２）μｍｏｌ·Ｌ－１，对照组为（２．５９１±０．１４２）μｍｏｌ·Ｌ－１，Ｐ＜０．０１］，膜微粘度增加［（０．４８１±０．０１４８）Ｐａ·ｓ，对照组为（０．２７１±０．０１３２）Ｐａ·ｓ，Ｐ＜０．０１］；红细胞ＬＰＯ与膜微粘度呈明显正相关（ｒ＝０．８９６，Ｐ＜０．０１）。提示氧自由基引发红细胞膜脂质过氧化引起的红细胞膜流动性降低在红细胞氧化损伤机制中起一定的作用。
To investigate the effect of erythrocyte membrane lipid peroxidation on erythrocyte membrane fluidity induced by oxygen free radicals in the mechanism of erythrocyte oxidative damage, the effect of oxygen free radical generating system (FeCl2 / ascorbate) on isolated normal erythrocytes was investigated by fluorescence polarization Method, thiobarbituric acid colorimetric determination of erythrocyte membrane microviscosity, erythrocyte lipid peroxides (LPO). The results showed that the LPO increased ([(6.181 ± 0.212) μmol·L-1 in erythrocytes and (2.591 ± 0.142) μmol·L-1 in erythrocytes) (0.481 ± 0.0148) Pa · s in the control group and (0.271 ± 0.0132) Pa · s in the control group, P <0.01] And membrane microviscosity was positively correlated (r = 0.896, P <0.01). Tip oxygen free radical induced erythrocyte membrane lipid peroxidation caused by reduced fluidity of erythrocyte membrane in erythrocyte oxidative damage mechanisms play a role.