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目的:观察肾虚质大鼠生长发育情况和海马N-甲基-D-天冬氨酸(NMDA)受体表达,探讨脑功能与肾藏志理论相关性。方法:采用“猫吓鼠”经典造模法造成先天不足加后天失养复合型肾虚质模型仔鼠,分为模型组、左归丸组、右归丸组,空白组产自正常孕鼠。选用补肾中药进行调理,观察仔鼠各项生长发育指标并检测NMDA受体表达。结果:(1)生长发育情况:与空白组相比,模型组体重下降,毛发稀疏、尿量增多、精神不振,各参数积分值有显著性差异(P<0.05);药物干预后情况明显改善,与模型组比较有统计学意义(P<0.05)。(2)NMDA受体表达:模型组亚基NR2A、NR2B灰度值较空白组低(P<0.05);用药后表达增强,灰度值变化有统计学意义(P<0.05)。结论:肾虚质大鼠在生长发育方面表现为体重下降、毛发不荣、尿量增多,精神不振,且生长发育低下状态与海马NMDA受体表达呈正相关;补肾药物可增强NMDA表达水平同时改善肾虚质大鼠低下的生长发育状况。
OBJECTIVE: To observe the growth and development of rats with kidney deficiency and the expression of N-methyl-D-aspartate (NMDA) receptor in hippocampus, and to explore the correlation between brain function and kidney occlusion theory. Methods: The model mice were randomly divided into model group, Zuogui pill group, Yougui pill group and blank group, which were born from normal pregnant rats . Use kidney medicine for conditioning, observe the growth and development of offspring indicators and detection of NMDA receptor expression. Results: (1) Growth and development: Compared with the blank group, the body weight of the model group decreased, the hair became sparse, the urine volume increased, and the spirit became sluggish, the integrals of each parameter had significant difference (P <0.05) , Compared with the model group was statistically significant (P <0.05). (2) NMDA receptor expression: The gray value of NR2A and NR2B in the model group was lower than that in the blank group (P <0.05). The expression of NMDA receptor was enhanced and the gray value was significantly changed after treatment (P <0.05). Conclusion: Kidney-deficient rats showed weight loss, hair loss, increased urine output, lack of energy, and poor growth and development and NMDA receptor expression in the hippocampus. Kidney-supplementing drugs can enhance the expression of NMDA and improve renal deficiency Low-quality rat growth and development.