挥发性盐基氮是动物性食品中蛋白质腐败变质的一个重要指标。目前常用的测定方法是半微量凯氏定氮法(下称原法),此法效果较好,但其测定装置要求严格、复杂,半微量定氮器尚不普及。为此,我们采用高沸点(相对水沸点而言)水浴法代替原法中的蒸气蒸馏,用简单的器材代替了原法的装置,经实验证明,效果满意。1 材料与方法1.1 原理及试剂同原法。1.2 仪器饱和盐水水浴锅;具塞长弯玻璃管(自制),挥发性盐基氮的发生吸收装置:取 Volatile base nitrogen is an important indicator of protein spoilage in animal foods. The commonly used determination method is semi-trace Kjeldahl method (hereinafter referred to as the original method), this method is better, but its measurement device requires strict and complex, semi-micro-nitrogen device is not yet universal. To this end, we use high boiling point (relative to the boiling point of water) instead of the original method of steam bath water distillation, with a simple device instead of the original device, the experiment proved satisfactory results. 1 Materials and Methods 1.1 Principles and reagents with the original method. 1.2 saturated brine water bath apparatus; stopper with curved glass tube (homemade), the occurrence of volatile nitrogen-based absorption device: take