论文部分内容阅读
目的:研究与修订前列舒乐泡腾片的质量标准。方法:化学鉴别法鉴别复方中药中的川牛膝和车前草;薄层色谱法鉴别中药复方中的蒲黄和黄芪。高效液相色谱法测定淫羊藿苷的含量,色谱柱为Polairs C18(150×4.6mm,5μm),流动相为乙腈-水(25∶75),检测波长270nm,流速1.0mL/min。结果:在薄层色谱中可鉴别出黄芪、蒲黄,无阴性干扰。淫羊藿苷在0.0493~0.4437μg范围内有良好的线性关系(r=0.9999),平均加样回收率(n=6)为99.33%(RSD=1.28%)。结论:该定性定量方法简便易行,重现性好,可作为前列舒乐泡腾片的质量标准基础。
Objective: To study and revise the quality standard of Qianlieshule effervescent tablets. METHODS: Chemical identification method was used to identify Chuan Bitch and Plantain in Compound Chinese Herbal Medicine; TLC was used to identify Puhuang and Astragalus in the compound of traditional Chinese medicine. The content of icariin was determined by HPLC. The column was Polaris C18 (150×4.6 mm, 5 μm). The mobile phase was acetonitrile-water (25:75). The detection wavelength was 270 nm and the flow rate was 1.0 mL/min. Results: Astragalus membranaceus and Pueraria can be identified in TLC without negative interference. There was a good linear relationship between icariin in the range of 0.0493-0.4437 μg (r=0.9999) and the average recovery (n=6) was 99.33% (RSD=1.28%). Conclusion: This qualitative and quantitative method is simple, easy to use, and has good reproducibility. It can be used as a basis for the quality standard of Qianlieshule effervescent tablets.