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目的观察马蹄香(Valeviana jatamansi Jones)对小鼠的急性毒性及对大鼠的亚急性毒性,以评价其安全性。方法给小鼠灌服5 000、7 500、10 000 mg/kg的马蹄香,对照组灌胃1%羧甲基纤维素钠(CMC),观察小鼠的中毒症状,记录小鼠的死亡数,采用Red-Munchen法计算半数致死量(LD50),并检查小鼠组织和脏器的病理变化;对大鼠分别灌服4 000、1 000和200 mg/kg的马蹄香,连续给药14 d,对照组灌胃CMC水溶液,给药后观察14 d,检测大鼠体重、脏器系数、血常规指标、血生化指标及组织病理变化情况。结果急性毒性试验结果显示,灌胃马蹄香后,小鼠短时间内出现活动减少,而后趋于正常,病理学检测未发现小鼠组织或脏器有明显异常改变,LD50>10 000 mg/kg;亚急性毒性试验结果显示,马蹄香各剂量组大鼠的增重、脏器系数、血常规指标、血生化指标及组织病理变化与对照组比较,差异均无统计学意义(P>0.05)。结论马蹄香具有较好的安全性。
Objective To observe the acute toxicity of Valeviana jatamansi Jones to mice and its subacute toxicity to evaluate its safety. Methods Mice were drenched with 500, 500, 000 and 10000 mg / kg of horseshoe incense, and the control group was given 1% sodium carboxymethyl cellulose (CMC). The mice were observed for signs of poisoning and the number of deaths , Red-Munchen method was used to calculate LD50, and pathological changes of tissues and organs of mice were examined. The rats were administered with 4,000, 1,000 and 200 mg / d. The control group was given intragastric administration of aqueous solution of CMC. After administration for 14 days, the body weight, organ coefficient, blood and blood biochemical indexes and histopathological changes were detected. Results The results of acute toxicity test showed that the activity of mice decreased after a short period of time and then became normal after the administration of Horseshoe. The pathological examination showed no obvious abnormal changes in the tissues or organs of mice. LD50> 10 000 mg / kg The results of subacute toxicity test showed that there was no significant difference in weight gain, organ coefficient, blood routine index, blood biochemical index and histopathological changes in each dose group compared with the control group (P> 0.05) . Conclusion Horseshoe incense has good safety.