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目的了解普萘洛尔、阿替洛尔、醋丁洛尔及噻吗洛尔对体外培养的血管内皮细胞增生及凋亡的影响。方法对人脐静脉血管内皮细胞进行体外培养,分别加入9种浓度的4种药物,设立阴性及阳性对照,采用cell counting kit-8(CCK-8)法检测细胞增生,Annexin-V FITC/PI双染法检测细胞凋亡。结果 1同一药物不同浓度之间抑制率比较:普萘洛尔和阿替洛尔结果一致,各个浓度之间抑制率P均<0.05;醋丁洛尔和噻吗洛尔结果一致,3.125μmol/L与12.5μmol/L及12.5μmol/L与50μmol/L抑制率P均>0.05,其余浓度间抑制率P均<0.05。2同一浓度的不同药物之间抑制率比较:在200、100、50μmol/L 3种浓度时,普萘洛尔与阿替洛尔、醋丁洛尔及噻吗洛尔抑制率比较P均<0.05,后3种药物抑制率比较P均>0.05;在25μmol/L浓度时,普萘洛尔与醋丁洛尔抑制率比较P均<0.05;其余药物抑制率比较P均>0.05。在12.500、6.250、3.125、1.562 5、0.781 3μmol/L这5种浓度中,同一浓度的不同药物抑制率比较P均>0.05。3对细胞凋亡的诱导:普萘洛尔、阿替洛尔与阴性对照组凋亡率比较P均<0.05;阿替洛尔、普萘洛尔、醋丁洛尔和噻吗洛尔与二氯化钴组凋亡率比较P均<0.05;而阿替洛尔、普萘洛尔、醋丁洛尔和噻吗洛尔4种药物凋亡率比较P均>0.05。结论 4种药物均可抑制细胞增生,普萘洛尔作用最强,且随着药物浓度增加抑制作用也明显增强;4种药物均可诱导细胞凋亡,但彼此之间无明显差异。
Objective To investigate the effects of propranolol, atenolol, acebutolol and timolol on the proliferation and apoptosis of cultured vascular endothelial cells. Methods Human umbilical vein endothelial cells were cultured in vitro. Nine different concentrations of four drugs were added to establish negative and positive controls. Cell counting kit-8 (CCK-8) was used to detect cell proliferation. Annexin-V FITC / PI Double staining for apoptosis. The results of 1 different concentrations of the same drug inhibition rates: propranolol and atenolol results were consistent, the inhibition rate between the various concentrations P <0.05; acebutolol and timolol results are consistent, 3.125μmol / L and 12.5μmol / L and 12.5μmol / L and 50μmol / L inhibition rate P> 0.05, the rest of the inhibition rate was P <0.05.2 Comparison of the same concentration of different drugs between the inhibition: at 200,100,50 μmol / L, the inhibitory rates of propranolol and atenolol, acebutolol and timolol were both P <0.05, and the inhibitory rates of the three drugs were both> 0.05. At 25μmol / L Concentration, propranolol and acebutolol inhibition rates were P <0.05; the rest of the drug inhibition rate were P> 0.05. Among the five concentrations of 12.500,6.250,3.125,1.562 5,0.781 3μmol / L, the inhibition rates of different drugs with the same concentration were all P> 0.05.3. Induction of apoptosis: Propranolol, atenolol P <0.05 compared with the negative control group; P <0.05 for atenolol, propranolol, acebutolol, timolol and cobalt dichloride; P <0.05; The apoptosis rates of the four drugs of lorol, propranolol, acebutolol and timolol were all> 0.05. CONCLUSION: Four kinds of drugs can inhibit cell proliferation, and propranolol has the strongest effect. With the increase of drug concentration, the inhibitory effect is also obviously enhanced. All four drugs can induce apoptosis, but no significant difference between them.