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[目的]探讨三氧化二砷(As2O3)作用于肝癌细胞后对其增殖抑制及线粒体跨膜电位(ΔΨm)的影响。[方法]用As2O3作用于体外培养人肝癌Mahlavu细胞,用MTT法检测As2O3对人肝癌Mahlavu细胞增殖的影响,并用流式细胞仪观察As2O3对人肝癌Mahlavu细胞ΔΨm的影响。[结果]MTT检测显示As2O3能明显抑制人肝癌Mahlavu细胞的增殖,并呈时间和剂量依赖性。流式细胞仪分析显示As2O3作用后的人肝癌Mahlavu细胞内ΔΨm明显降低(P<0.01),并呈时间和剂量依赖性。[结论]As2O3可降低人肝癌Mahlavu细胞ΔΨm,并可由此诱导细胞凋亡而抑制其增殖。这可能也是As2O3诱导肝癌Mahlavu细胞凋亡的机制之一。
[Objective] To explore the effect of arsenic trioxide (As2O3) on the proliferation inhibition and mitochondrial transmembrane potential (ΔΨm) of hepatocellular carcinoma cells. [Methods] Human kidney cancer (Mahlavu) cells were treated with As2O3. MTT assay was used to detect the effect of As2O3 on the proliferation of human liver cancer cell line Mahlavu. Flow cytometry was used to observe the effect of As2O3 on ΔΨm of human liver cancer cell line Mahlavu. [Results] MTT assay showed that As2O3 could significantly inhibit the proliferation of human liver cancer Mahlavu cells in a dose- and time-dependent manner. Flow cytometry analysis showed that ΔΨm in the human liver cancer cell Mahlavu after As2O3 treatment was significantly decreased (P<0.01), and was time and dose dependent. [Conclusion] As2O3 can reduce the ΔΨm of human liver cancer Mahlavu cells, and can induce apoptosis and inhibit its proliferation. This may be one of the mechanisms of As2O3-induced apoptosis of liver cancer Mahlavu cells.