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氨基酸的分析方法应用最普遍的是离子交换色谱法。在-SH存在下,邻苯二甲醛(OPA)与伯氨基在柱后反应产生荧光物质进行氨基酸的检测是一种有效的微量分析法。而亚氨酸则须先用次氯酸钠氧化再与OPA反应。整个系统如图1如示。我们应用此法分析了谷物的总氨基酸和茶叶中的游离氨基酸含量。
The most commonly used method of amino acid analysis is ion exchange chromatography. In the presence of -SH, phthalaldehyde (OPA) and primary amino in the post-column reaction of fluorescent substances for amino acid detection is an effective microanalysis. Imino acids and then have to use sodium hypochlorite oxidation and then OPA reaction. The entire system shown in Figure 1 as shown. We applied this method to analyze the total amino acids in cereals and free amino acids in tea.