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线粒体染色传统的方法较多,如Altmann酸性复红法,Benda结晶紫法、Baker酸性氧化苏木素法及Heidenhain铁苏木精法等,这些都是较好的方法,但过程比较复杂,铁苏木精染色时间长,分色不易掌握。作者试用LuxoL快兰染线粒体效果特好,这是由于线粒体中蛋白质磷脂含量很高,有大量羟基、磷酸基负电子基团、与LuxoL快兰极易结合、故显示线粒体形态清晰、色泽分明,颜色经久不退,能较长期保存,整个技术过程简便。
There are many traditional methods of mitochondrial staining, such as Altmann acid complex red method, Benda crystal violet method, Baker acidic oxidized hematoxylin method and Heidenhain iron hematoxylin method, these are better methods, but the process is more complex, iron hematoxylin Fine dyeing time, color separation is not easy to grasp. The author tried LuxoL fast blue dye mitochondrial effect is particularly good, this is due to the high content of protein phospholipid mitochondria, a large number of hydroxyl, phosphate-based electronic group, and LuxoL fast Lan extremely easy to combine, it shows a clear mitochondria, color, Long lasting color, can be more long-term preservation, the entire technical process is simple.