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从大果紫檀根瘤中分离到一株慢生型根瘤菌DG,通过其形态特征观察、保守基因序列分析以及生物学特性研究等方面进行种类鉴定,通过结瘤试验了解DG与降香黄檀的共生关系,并探讨其共生固氮能力.16S rRNA、nifH和dnaK系统发育结果表明,该菌株与Bradyrhizobium elkanii同源性最高.菌株DG与B,elkanii USDA76T在碳源利用、抗生素抗性、耐受NaCl等生物学特性上基本一致,而且还能在pH 10.0下良好生长.结瘤试验显示,菌株DG能在降香黄檀根部结瘤;接种DG的降香黄檀幼苗植株的株高、干重以及全氮含量(P<0.05)显著高于对照处理,表明DG能与降香黄檀形成良好的共生关系,且具有高效的固氮能力.图4表2参27
A slow-growing Rhizobium DG was isolated from the root nodules of P. grandis. The morphological characteristics, conserved gene sequences and biological characteristics of DG were identified. The nodulation test was used to understand DG and Rhodiola rosea Symbiotic relationship and its symbiotic nitrogen fixation ability.The phylogenetic analysis of 16S rRNA, nifH and dnaK showed that this strain had the highest homology with Bradyrhizobium elkanii.The results showed that the strains DG and B, elkanii USDA76T had the highest utilization of carbon sources, antibiotic resistance and tolerance to NaCl And other biological characteristics are basically the same, but also good growth at pH 10.0 nodulation test showed that the strain DG can root nodulation in Dalbergia henryi; inoculated DG of Dalbergia solani seedlings plant height, dry weight And total nitrogen (P <0.05) were significantly higher than the control treatment, indicating that the DG and Dalbergia can form a good symbiotic relationship, and has an efficient nitrogen fixation capacity.