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目的研究新型多酸化合物(POM-2)体外抗乙型肝炎病毒(HBV)的活性和体外毒性。方法采用实时荧光定量PCR分析受试多酸化合物对HepG2.2.15细胞外HBV DNA的抑制率,计算其50%抑制浓度(IC50)值和90%抑制浓度(IC90)值;采用乙型肝炎病毒e(s)抗原诊断试剂盒测定细胞培养上清液中HBeAg、HBsAg的含量,测定受试多酸化合物对HBeAg、HBsAg分泌的抑制率;采用MTT法测定受试多酸化合物对HepG2.2.15细胞的毒性,计算其50%致死浓度(CC50)值。结果新型多酸化合物对HepG2.2.15细胞外HBV DNA的50%抑制浓度(IC50)和90%抑制浓度(IC90)分别为13.42 mg.L-15、9.47 mg.L-1。各浓度实验组对HBeAg、HBsAg分泌的抑制率均高于对照组(P<0.05)。受试多酸化合物对HepG2.2.15细胞的50%致死浓度(CC50)值为2899.21 mg.L-1。结论新型多酸化合物(POM-2)体外毒性低,对HepG2.2.15细胞外HBV DNA及HBeAg、HBsAg的分泌具有较好的抑制作用。
Objective To study the activity and in vitro toxicity of POM-2 against hepatitis B virus (HBV) in vitro. Methods The inhibitory rates of extracellular HBV DNA of HepG2.2.15 cells were tested by real-time fluorescence quantitative PCR. The 50% inhibitory concentration (IC50) and the 90% inhibitory concentration (IC50) were calculated. The hepatitis B virus e (s) antigen diagnostic kit for the determination of HBeAg and HBsAg in cell culture supernatant to determine the inhibition of HBeAg and HBsAg secretion by the test polyacid compound. The inhibitory effect of the tested polyacid compound on HepG2.2.15 cells Toxicity, calculate its 50% lethal concentration (CC50) value. Results The 50% inhibitory concentration (IC50) and the 90% inhibitory concentration (IC90) of novel polyacid compounds on HepG2.2.15 extracellular HBV DNA were 13.42 mg.L-15,9.47 mg.L-1, respectively. The inhibitory rates of HBeAg and HBsAg secretion in experimental group were higher than those in control group (P <0.05). The 50% lethal concentration (CC50) of the tested polyacid compounds on HepG2.2.15 cells was 2899.21 mg.L-1. Conclusion The new polyoxometalate compound (POM-2) has low toxicity in vitro and good inhibitory effect on the secretion of HBV DNA, HBeAg and HBsAg in HepG2.2.15 cells.