论文部分内容阅读
目的:观察不同配比甘草-芫花煎剂对肝癌腹水模型小鼠的干预影响,并探讨其作用机制。方法:取昆明种雄性小鼠135只,随机分为正常组、模型组、芫花单煎组、芫花-甘草(1∶0.25,1∶0.5,1∶1,1∶2,1∶4)组和甘草单煎组。除正常组外,其他组小鼠腹腔均接种肝癌腹水瘤细胞系H22,并分别灌胃给药,正常组和模型组给予等容积生理盐水。12 d后分别测量各组小鼠的腹水量、腹围和体重及肝肾指数,蛋白质免疫印迹(Western blot)法测定肾脏组织中水通道蛋白2(AQP2)和肾加压素受体(V2R)的蛋白表达,生化法测定小鼠血清中丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),尿素氮(BUN)和尿肌酐(Cr)的水平。结果:与正常组比较,模型组小鼠的腹水量、腹围、体重、肝指数及血清中ALT,AST,BUN,Cr水平均显著升高(P<0.01),肾指数显著降低(P<0.01),AQP2,V2R蛋白的表达显著升高(P<0.01)。与模型组比较,芫花单煎组和芫花-甘草1∶0.5,1∶1和1∶2组均能显著降低小鼠的腹水量和体重(P<0.05,P<0.01);芫花单煎组和芫花-甘草1∶1组同时能显著降低小鼠的腹围(P<0.05,P<0.01),降低AQP2,V2R蛋白的表达(P<0.05,P<0.01)。与芫花单煎组比较,芫花-甘草1∶1组能显著降低小鼠的腹水量、腹围和体重(P<0.05,P<0.01),降低AQP2,V2R的表达(P<0.05),芫花-甘草1∶4组则使其显著升高(P<0.05,P<0.01)。结论:不同比例甘草的配伍能增强或抵消芫花抗小鼠肝癌腹水的作用,且相关变化可能与其有效调节了肾组织内的AQP2和V2R蛋白表达有关。
Objective: To observe the different proportion of licorice - Daphne flower decoction on liver cancer ascites model mice intervention and explore its mechanism of action. Methods: 135 male Kunming mice were randomly divided into normal group, model group, Daphne genkwa single decoction group, Daphne genkwa - licorice (1: 0.25,1: 0.5,1: 1: ) Group and licorice single fried group. In addition to the normal group, the mice in other groups were inoculated intraperitoneally with hepatoma ascites tumor cell line H22, and were given intragastric administration, normal group and model group were given equal volume of normal saline. After 12 days, the ascites, abdominal circumference and body weight, liver and kidney index, and AQP2 and vasopressin receptor (V2R) were measured by Western blot in each group. ), And the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and urinary creatinine (Cr) in serum of mice were determined by biochemical method. Results: Compared with the normal group, the ascites, abdominal circumference, body weight, liver index and serum ALT, AST, BUN and Cr in the model group were significantly increased (P <0.01) 0.01). The expression of AQP2 and V2R protein were significantly increased (P <0.01). Compared with the model group, 1: 0.5, 1: 1 and 1: 2 groups of Daphne gengramisi decoction and Daphne genkwa-licorice could significantly reduce the ascites volume and body weight in mice (P <0.05, P <0.01) The single-decoction group and Daphne genkwa-licorice 1: 1 group significantly reduced the abdominal circumference (P <0.05, P <0.01) and decreased the expressions of AQP2 and V2R protein (P <0.05, P <0.01). Compared with Daphne genkwa single decoction group, the Daphne genkwa 1: 1 group significantly reduced the ascites, abdominal circumference and body weight (P <0.05, P <0.01), and decreased the expression of AQP2 and V2R , Daphne genkwa - licorice 1: 4 group was significantly increased (P <0.05, P <0.01). Conclusion: The compatibility of different proportion of licorice can increase or offset the effect of Daphne genkwa against mouse hepatocellular carcinoma ascites, and the related changes may be related to its regulation of AQP2 and V2R protein expression in renal tissue.