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200813 乳腺癌染色体3p~(14～24)区域不同位点杂合缺失的初步分析/王洪义…//中国肿瘤临床.-1999,26(10).-736～738 用PCR-限制性长度片段多态性分析法,对56例原发性乳腺癌3号染色体短臂3p~(14-24)区域杂合缺失(LOH)进行分析。结果:在染色体3P~(14)、3P~(21)、3P~(24)3个位点上LOH发生的频率分别为4.2%(1/24)、0%(0/9)和39.5%(15/38),3p~(24)位点LOH发生的频率最高,说明该位点有乳腺癌相关抑制基因存在的可能性。分析3P~(24)位点的LOH与临床分期、淋巴结转移和ER、PR的相关关系,发现在8例Ⅰ期乳腺癌信息个体中,该位点LOH全部阴性,在Ⅱ、Ⅲ期病例LOH阳性率分别为52.2%(12/23)和42.8%(3/7);在腋淋巴结转移和无转移组中分别为66.7%(12/18)、15%(3/20),两组差异显著;ER或PR阴性者也较阳性者有较高的LOH发生率。说明在Ⅱ、Ⅲ期乳腺癌以及淋巴结阳性、ER或PR阴性的肿瘤中,该位点的缺失更频繁,提示3P~(24)位点基因缺失与病程进展快、侵袭性程度高和预后差的肿瘤有关。认为
200813 Chromosome 3p ~ (14 ~ 24) region at different sites of heterozygous loss of a preliminary analysis / Wang Hongyi ... / / China Cancer Clinical.-1999,26 (10) .- 736 ~ 738 PCR-restriction fragment length To analyze the heterozygous deletion (LOH) in the 3p ~ (14-24) region of the short arm of chromosome 3 in 56 cases of primary breast cancer. Results: The frequency of LOH occurred at 4.2% (1/24), 0% (0/9), and 39.5% at chromosome 3P ~ (14), 3P ~ (21) and 3P ~ (24) (15/38). The frequency of LOH occurred at the 3p ~ (24) site was the highest, indicating that there was a possibility of the presence of breast cancer related suppressor gene in this locus. The correlation between LOH at 3P ~ (24) site and clinical stage, lymph node metastasis and ER, PR was analyzed. It was found that all the LOH in this group were negative in 8 cases of stage I breast cancer. The positive rates were 52.2% (12/23) and 42.8% (3/7) respectively. The positive rates were 66.7% (12/18) and 15% (3/20) in axillary lymph node metastasis and non-metastasis group, respectively Significant; ER or PR-negative than those who have a higher incidence of LOH. These results suggest that the deletion of this locus is more frequent in stage II and III breast cancer and lymph node positive, ER or PR negative tumors, suggesting that the gene deletion at 3P ~ (24) locates with fast progression, high invasiveness and poor prognosis Related to the tumor. think