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为选择出马尾松松材线虫抗性候选木,安徽省松材线虫抗性育种中心从2003~2005年进行了1次接种测定和2次接种测定。2次测定共对298个家系8、035株苗木进行了接种,接种后的平均生存率分别为57.0%、92.6%,不同的接种环境差异明显反映到接种的结果上。从2次测定的合格苗中,2004年选择出84个家系3、94株,2005年选择出157个家系、785株,合计241个家系1、178株苗木作为抗性候选木。此外,该中心利用从广西、广东等地引进的种子进行接种测定,独自选择出10个家系3、1株抗性候选木。共选择出251个家系1、209株抗性候选木。尝试利用2次测定的家系存活率对各抗性候选木的抗性进行评价,结果表明有13个家系的候选木具有强抗性。利用同工酶分析,2004年将选择出来的3个地域的抗性候选木进行基因频率的计算,试图找出地域间的变异。结果表明,作为遗传多样性指数的杂合体率(He),虽然比湖北省的8个群体的平均值(0.262)低,但是比现在报道的松属的平均值(0.157)高,因此不能得出遗传多样性很低的结论。
In order to select the wood of P. massoniana resistance candidate, Anhui Pine Wood Nematode Resistance Breeding Center conducted one inoculation and two inoculation tests from 2003 to 2005. In total, 8,035 seedlings of 298 pedigrees were inoculated twice after the inoculation. The average survival rates after inoculation were 57.0% and 92.6%, respectively. The differences in the inoculation environment were clearly reflected in the inoculation results. From the qualified samples of two determinations, 3,94 84 families were selected in 2004 and 157 families and 785 strains were selected in 2005. A total of 1,178 seedlings of 241 pedigrees were selected as candidate plants for resistance. In addition, the center made use of seeds introduced from Guangxi, Guangdong and other places for inoculation, and independently selected 10 pedigrees 3 and 1 resistant candidate wood. Select a total of 251 families of 1,209 resistant candidate wood. Attempts to evaluate the resistance of each resistant candidate wood using the measured second-line survival rate showed that 13 families of candidate wood were highly resistant. Using isoenzyme analysis, in 2004, we selected three candidate resistance loci for gene frequency calculation in order to find out the variation among regions. The results showed that the heterozygote rate (He) as a genetic diversity index was lower than the average (0.262) of 8 populations in Hubei Province but higher than the average (0.157) of the Pinus now reported, Conclusions are low genetic diversity.