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以80个来自欧洲白桦(Betula pendula Roth)×中国白桦(Betula platyphylla Suk)的F1个体为作图群体。利用2个亲本和10个F1个体对1,200个10bp的随机寡核苷酸引物进行筛选,确定了208个多态性引物。利用RAPD标记,按照拟测交的作图策略,分别构建了欧洲白桦和中国白桦的分子标记连锁图谱。对2个亲本和80个F1代作图群体进行随机扩增,共获得了364个多态性位点。χ2检验结果表明有307个位点符合1∶1分离的拟测交分离,26个位点符合3∶1分离,31个位点属偏分离位点。拟测交位点中有145个位点来自欧洲白桦,有162个位点来自中国白桦。利用2点连锁分析,欧洲白桦中的145个连锁标记构成了14个不同的连锁群(4个以上标记),6个三连体和6个连锁对,37个为非连锁位点,连锁标记覆盖的总图距为955.6cM(centimorgan),平均图距14.9cM。而来自中国白桦的162个标记构成了15个连锁群(4个以上标记),4个三连体和6个连锁对,21个为非连锁位点,连锁标记覆盖的总图距为1,545.8cM(centimorgan),平均图距15.2cM。该图谱的建立为进一步将两个图谱整合为一个高密度图谱及重要基因的定位奠定了基础。
Eighty F1 individuals from Betula pendula Roth × Betula platyphylla Suk were used as a mapping population. A total of 208 polymorphic primers were identified by screening 1,200 random 10 bp oligonucleotide primers using 2 parents and 10 F1 individuals. RAPD markers were used to construct molecular linkage map of Betula platyphylla and Betula platyphylla respectively according to the mapping strategy to be tested. A total of 364 polymorphic loci were obtained by random amplification of two parents and 80 F1 generation. The results of χ2 test showed that 307 loci were fit for 1: 1 segregation, 26 loci were 3: 1 segregation, and 31 loci were partial segregation sites. In the proposed site, 145 loci were from European white birch, and 162 loci were from Chinese white birch. Using 2-point linkage analysis, 145 linkage markers in European white birch constitute 14 different linkage groups (more than 4 markers), 6 triplets and 6 linkage pairs, 37 are non-linkage sites and the linkage markers The total coverage is 955.6 cM (centimorgan), with an average of 14.9 cM. While 162 markers from Chinese birch constitute 15 linkage groups (more than 4 markers), 4 triplets and 6 linkage pairs, 21 are non-linkage sites, and the total distance covered by linkage markers is 1,545.8 cM centimorgan average distance 15.2cM. The establishment of this map laid the foundation for the further integration of the two maps into a high-density map and the location of important genes.