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国产沉香为瑞香科(Thymelaeaceae)植物白木香(Aquilaria sinensis(Lour.)Gilg)含有树脂的木材。白木香树体只有在受到伤害等胁迫,才在伤口周围的木材内产生倍半萜等沉香类物质,但是伤害如何诱导沉香倍半萜生物合成途径至今尚未揭示。为揭示伤害诱导白木香结香的分子机制,本研究应用RNA测序技术对机械伤害后的白木香木质部转录组进行测序,研究其基因表达谱,挖掘其功能基因。本研究共获得40 295条表达序列标签(express sequence tags,EST),平均长度305 bp。经序列合并拼接,获得22 095条单基因簇(unigene)。通过核苷酸和蛋白质序列等方面的同源性分析,表明其中61.6%(13 611条)与其他生物的已知基因具有不同程度的同源性。通过功能分类研究(GeneOntology)和代谢途径分析(KEGG)获得参与沉香倍半萜合成的序列26条(编码7个关键酶)。这些基因的发现为研究沉香倍半萜化合物的生物合成途径及伤害诱导沉香形成的分子机制提供了基础数据。
The Chinese-made incense is a wood containing resin from Aquilaria sinensis (Lour.) Gilg, a plant belonging to the Thymelaeaceae family. Baizhu fragrant body only in the coercion of such damage, only in the wood around the wound produce sesquiterpene and other agarics, but how injury induced sesquiterpenoid biosynthetic pathway has not yet been revealed. In order to reveal the molecular mechanism of injury-inducing almond blossom, RNAi was used to sequence the transcriptome of xylostella xylostella after mechanical injury, to study its gene expression profile and to explore its functional genes. A total of 40 295 express sequence tags (ESTs) were obtained in this study, with an average length of 305 bp. After the sequence merging splicing, 22 095 single gene clusters (unigene) were obtained. Homology analysis of nucleotide and protein sequences revealed that 61.6% (13 611) of them had different degrees of homology with known genes of other organisms. Twenty-six sequences (encoding seven key enzymes) involved in the synthesis of basil sesquiterpenes were obtained by GeneOntology and metabolic pathway analysis (KEGG). The discovery of these genes provided the basic data for studying the biosynthetic pathway of the sesquiterpenoids and the molecular mechanism of injury-induced formation of agouti.