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目的了解镇江市活禽市场等外环境样本中禽流感病毒的动态分布情况,为防治人感染禽流感提供科学依据。方法采用实时荧光定量PCR法对2014年-2015年镇江市外环境标本(清洗禽类的污水、笼具表面及禽类粪便等)进行禽流感病毒核酸检测,阳性者进一步检测H5、H7N9、H9亚型核酸,监测数据用SPSS 19.0软件进行统计分析。结果共采集外环境标本1 058份,甲型通用病毒核酸阳性标本共284份,总阳性率为26.84%;H5、H7N9、H9亚型和混合感染的检出率分别为5.10%、17.67%、3.02%和1.04%。外环境标本中禽流感核酸阳性率有明显的季节性,冬季、春季高于夏季。禽流感病毒检出率最高的标本为清洗禽类的污水(41.57%),最低为鸡食(3.23%)。结论镇江市城乡活禽市场与宰杀加工场所等外环境中有H5、H7N9、H9等多种亚型禽流感病毒存在,建议继续加强外环境禽流感实时监测,对发现禽流感病毒变异、预防控制禽流感具有重要的意义。
Objective To understand the dynamic distribution of avian influenza virus in external environmental samples such as live poultry market in Zhenjiang and provide a scientific basis for prevention and control of bird flu. Methods The real-time fluorescence quantitative PCR was used to detect the avian influenza virus nucleic acid in the external environment samples of Zhenjiang from 2014 to 2015 (sewage, cage surface and poultry faeces, etc.). Positive samples were further tested for H5, H7N9 and H9 subtypes Nucleic acid, monitoring data using SPSS 19.0 software for statistical analysis. Results A total of 1 058 environmental samples were collected. A total of 284 positive samples of A virus were obtained, the total positive rate was 26.84%. The detection rates of H5, H7N9, H9 subtypes and mixed infection were 5.10%, 17.67% 3.02% and 1.04%. The positive rate of avian influenza nucleic acid in external environment specimens was obviously seasonal, which was higher in winter and spring than in summer. The highest detection rate of avian influenza virus for the cleaning of birds, sewage (41.57%), the lowest chicken food (3.23%). Conclusions H5, H7N9, H9 and many other avian influenza viruses exist in the external environment such as live market and slaughterhouse in urban and rural areas of Zhenjiang City. It is suggested that real-time monitoring of avian influenza in external environment should be strengthened and the detection of avian influenza virus variation, prevention and control Bird flu is of great significance.