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通过动态观察血浆及支气管肺泡灌洗液(BALF)中PLAP的变化,探讨烟雾吸入所致肺泡Ⅰ型细胞损害及其与肺损伤发生发展的关系,采用大鼠烟雾吸入伤模型,分别检测了正常对照及致伤2,6,12和24小时动物的动脉血气、肺水量、BALF中总蛋白和白蛋白含量,BALF和血浆中PLAP含量,并作了病理检查。结果表明:动物伤后出现急性呼吸衰竭和严重肺水肿,BALF中总蛋白及白蛋白含量明显升高;血浆及BALF中PLAP水平亦显著增加,且与BALF中蛋白含量的改变相关显著;病理检查亦见严重肺泡Ⅰ型细胞损害。提示PLAP不仅可作为肺泡Ⅰ型细胞损害的标志,而且与烟雾吸入伤后肺泡一毛细血管膜通透性增加有密切关系。
By observing the changes of PLAP in plasma and bronchoalveolar lavage fluid (BALF) dynamically, the relationship between the injury of alveolar type 1 cells induced by smoke inhalation and the occurrence and development of lung injury was discussed. The model of smoke inhalation injury in rats was used to detect normal Arterial blood gas, pulmonary water volume, total protein and albumin content in BALF, PLAP content in BALF and plasma were detected and pathologically examined in control and injured animals at 2, 6, 12 and 24 hours. The results showed that acute respiratory failure and severe pulmonary edema appeared after acute injury, the content of total protein and albumin in BALF were significantly increased, PLAP level in plasma and BALF was also significantly increased, which was significantly correlated with the change of protein content in BALF. See also Severe alveolar type 1 cell damage. These results suggest that PLAP can be used not only as a marker of alveolar type 1 cell damage but also closely related to the increase of alveolar-capillary membrane permeability after smoke inhalation injury.